The bus was taking Easter pilgrims from Botswana to a town in Limpopo.
A bus crash in South Africa's northern province of Limpopo resulted in 45 deaths and one serious injury, South Africa's Department of Transport said on Thursday.
The driver lost control and collided with barriers on a bridge near Mamatlakala, causing the bus to go over the bridge and hit the ground, catching fire, according to a statement by the transport department.
The bus was taking Easter pilgrims from Botswana, a landlocked country in Southern Africa to Moria, a town in Limpopo, it added.
South African President Cyril Ramaphosa sent his condolences to Botswana and pledged support to the country, his office said in a statement.
Only an 8-year-old survived the crash and was receiving medical attention at a nearby hospital, Limpopo's Department of Transport said in a separate statement.
Some bodies were burned beyond recognition while others were trapped inside the debris or scattered on the scene, the provincial department said.